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Metagenomics of Bolidophyceae in Plankton and Ice of the White Sea

T. A. Belevich1, L. V. Ilyash1, I. A. Milyutina2, M. D. Logacheva2,3, and A. V. Troitsky2*

1Lomonosov Moscow State University, Faculty of Biology, 119991 Moscow, Russia

2Lomonosov Moscow State University, Belozersky Institute of Physico-Chemical Biology, 119991 Moscow, Russia; E-mail: bobr@belozersky.msu.ru

3Kazan Federal University, Institute of Fundamental Biology and Medicine, Laboratory of Extreme Biology, 420012 Kazan, Russia

* To whom correspondence should be addressed.

Received July 31, 2017; Revision received September 7, 2017
The molecular diversity of poorly studied algae of Bolidophyceae class was first estimated by Illumina sequencing of V4 region of 18S rRNA gene in ice, under-ice water and summer water of the subarctic White Sea. We used two clustering thresholds – 93 and 97% – and revealed 31 phylotypes of Bolidophyceae. Triparma pacifica and T. strigata were identified to species level. The association of individual phylotypes to certain biotopes (ice or plankton) and stages of seasonal succession (under ice or summer plankton) has been established. Some phylotypes are found in different biotopes and over a wide temperature range. Due to changing their genetic composition, Bolidophyceae are a constant component of the photoautotrophic plankton and ice communities.
KEY WORDS: metagenomic analysis, picophytoplankton, White Sea, Bolidophyceae, Triparma pacifica, Triparma strigata

DOI: 10.1134/S0006297917120136