2Lomonosov Moscow State University, Faculty of Chemistry, 119899 Moscow, Russia
3State Research Institute of Chemistry and Technology of Organoelement Compounds, 105118 Moscow, Russia
* To whom correspondence should be addressed.
Received January 19, 2018; Revision received March 20, 2018
Derivatization of the natural flavonoid dihydroquercetin with p-aminobenzoic acid was carried out in an ethyl acetate/citric buffer biphasic system using laccase from the fungus Trametes hirsuta. The main reaction product yield was ~68 mol %. The product was characterized by 1H NMR, 13C NMR, and liquid chromatography-mass spectroscopy, and its structure was elucidated. The reaction product affected viability of cultured human rhabdomyosarcoma cells (RD cell line) in a dose-dependent manner and, therefore, can be of interest to pharmaceutical industry.
KEY WORDS: dihydroquercetin, p-aminobenzoic acid, laccase, biotransformation, 1H and 13C NMR spectra, proteomics