New Sorbent on the Basis of Covalently Immobilized Lysozyme for Removal of Bacterial Lipopolysaccharide (Endotoxin) from Biological Fluids

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P. A. Levashov^1,2,a*, D. A. Matolygina^1,2, E. D. Ovchinnikova³, I. Yu. Adamova⁴, O. A. Dmitrieva³, A. V. Nuzhdina², N. S. Pokrovsky^2,5, and N. L. Eremeev¹

¹Lomonosov Moscow State University, Faculty of Chemistry, 119234 Moscow, Russia

²Bauman Moscow State Technical University, Interindustry Engineering Center for Composite Materials, 105005 Moscow, Russia

³National Medical Research Center of Cardiology, Institute of Experimental Cardiology, Ministry of Healthcare of the Russian Federation, 121359 Moscow, Russia

⁴POKARD Ltd, 121359 Moscow, Russia

⁵Lomonosov Moscow State University, Faculty of Fundamental Medicine, 119991 Moscow, Russia

^* To whom correspondence should be addressed.

Received July 23, 2018; Revised September 9, 2018; Accepted September 9, 2018

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It was demonstrated for the first time that immobilized lysozyme can efficiently remove Escherichia coli and Pseudomonas aeruginosa lipopolysaccharides (endotoxins) from solutions. Experimentally confirmed sorption capacity for the developed sorbent was at least 400 ng of endotoxin per ml sorbent. The new sorbent is compatible with the whole human blood and can be potentially used in extracorporeal therapy in the treatment of sepsis.

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KEY WORDS: endotoxin sorption, sepsis, immobilized lysozyme

DOI: 10.1134/S0006297919010048

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