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Extracellular MicroRNAs and Mitochondrial DNA as Potential Biomarkers of Arrhythmogenic Cardiomyopathy

A. A. Khudiakov1,a*, N. A. Smolina1, K. I. Perepelina1,2, A. B. Malashicheva1,2, and A. A. Kostareva1

1Almazov National Medical Research Center, 197341 St. Petersburg, Russia

2St. Petersburg State University, 199034 St. Petersburg, Russia

* To whom correspondence should be addressed.

Received October 16, 2018; Revised December 3, 2018; Accepted December 3, 2018
Differential diagnosis of arrhythmogenic cardiomyopathy (ACM) during the disease latent phase is a challenging clinical problem that requires identification of early ACM biomarkers. Because extracellular nucleic acids are stable, specific, and can be easily detected, they can be used as reliable biomarkers of various diseases. In this study, we analyzed the levels of extracellular microRNAs and mitochondrial DNA in the conditioned medium collected from cardiomyocytes differentiated from induced pluripotent stem cells of ACM patients and healthy donor. Several microRNAs were expressed differently by the affected and healthy cardiomyocytes; therefore, they could be considered as potential ACM biomarkers.
KEY WORDS: arrhythmogenic cardiomyopathy, cardiomyocytes, microRNA, mitochondrial DNA, induced pluripotent stem cells

DOI: 10.1134/S000629791903009X