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REVIEW: Live-Cell Super-resolution Fluorescence Microscopy

A. S. Mishin1,a* and K. A. Lukyanov1

1Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia

* To whom correspondence should be addressed.

Received August 3, 2018; Revised September 5, 2018; Accepted September 5, 2018
Super-resolution fluorescence microscopy (nanoscopy) enables imaging with a spatial resolution much higher than the diffraction limit of optical microscopy. However, the methods of fluorescence nanoscopy are still poorly suitable for studying living cells. In this review, we describe some of methods for nanoscopy and specific fluorescent labeling aimed to decrease the damaging effects of light illumination on live samples.
KEY WORDS: super-resolution fluorescence microscopy, STED, RESOLFT, single molecule localization, protein labeling, photoconversion

DOI: 10.1134/S0006297919140025