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REVIEW: Single Cell Proteogenomics – Immediate Prospects

S. A. Moshkovskii1,2,a*, A. A. Lobas3, and M. V. Gorshkov3

1Pirogov Russian National Research Medical University, 117997 Moscow, Russia

2Orekhovich Institute of Biomedical Chemistry, 119121 Moscow, Russia

3Talrose Institute for Energy Problems of Chemical Physics, Semenov Federal Research Center of Chemical Physics, Russian Academy of Sciences, 119334 Moscow, Russia

* To whom correspondence should be addressed.

Received October 15, 2019; Revised November 11, 2019; Accepted November 11, 2019
Recent technical advances in genomic technology have led to the explosive growth of transcriptome-wide studies at the level of single cells. The review describes the first steps of the single cell proteomics that has originated soon after development of transcriptomics methods. The first studies on the shotgun proteomics of single cells that used liquid chromatography/mass spectrometry have been already published. In these works, the cells were separated by the methods used in transcriptomics studies (e.g., cell sorting) and analyzed by modified mass spectrometry with tandem mass tags. The new proteogenomics approach involving integration of single cell transcriptomics and proteomics data will provide better understanding of the mechanisms of cell interactions in normal development and disease.
KEY WORDS: proteomics, transcriptomics, proteogenomics, single cell analysis, tandem mass tag (TMT), mass spectrometry

DOI: 10.1134/S0006297920020029