REVIEW: Myeloperoxidase as a Regulator of Cell Signaling under Oxidative/Halogenative Stress

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Irina V. Gorudko¹, Daria V. Grigorieva¹, Veronika E. Reut¹, Grigory G. Martinovich¹, Alexey V. Sokolov^2,3, and Oleg M. Panasenko^3,a*

¹Belarusian State University, Minsk, 220030, Belarus

²Federal State Budgetary Institution “A. A. Smorodintsev Research Institute of Influenza” of the Ministry of Health of the Russian Federation, 197376 Saint Petersburg, Russia

³Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical-Biological Agency, 119435 Moscow, Russia

^* To whom correspondence should be addressed.

Received: August 25, 2025; Revised: September 16, 2025; Accepted: September 29, 2025

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This review has considered the mechanisms of intracellular and intercellular signaling regulation by myeloperoxidase (MPO), an enzyme in neutrophil azurophilic granules, during oxidative/halogenative stress and inflammation development. The stages of enzyme functioning and the formation of reactive halogen species are described. The functioning of MPO and production of reactive halogen species are shown to depend on the activity of NADPH oxidase; the role of NADPH oxidase and reactive oxygen species in the regulation of MPO function is discussed. Particular attention is focused on the role of biological molecules modified by reactive halogen species in modulating NADPH oxidase activity, exocytosis of granular proteins, NETosis, and other neutrophil functions, based on the principle of positive feedback. A special feature of the review is the discussion of the non-canonical function of MPO, namely its signaling role in the regulation of cellular processes, which is not associated with the catalytic activity of the enzyme.

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KEY WORDS: myeloperoxidase, cell signaling, reactive halogen species, halogenative stress, reactive oxygen species, NADPH oxidase, oxidative stress

DOI: 10.1134/S0006297925603788

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