REVIEW: Yeast Engineering for Antioxidant Production

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Yulia S. Panina^1,a*, Sergey A. Bruskin¹, Svetlana O. Avdoshina¹, Liliya G. Maloshenok¹, Tigran Yuzbashev², and Evgeniya Yuzbasheva^3,b*

¹N. I. Vavilov Institute of General Genetics, Russian Academy of Sciences, 119991 Moscow, Russia

²Plant Sciences and the Bioeconomy, Rothamsted Research, Harpenden, AL5 2JQ, UK

³BioMediCan Inc., Fremont, CA 94538, USA

^* To whom correspondence should be addressed.

Received: September 14, 2025; Revised: September 29, 2025; Accepted: October 21, 2025

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This article reviews biosynthesis of the valuable fat-soluble compounds with antioxidant activity, in particular vitamin E isomers and carotenoids, in yeast cells. Main genetic engineering approaches to increase microbial production of these substances are described. The main innovative strategies for subcellular separation of synthesis, storage, and recovery of lipophilic compounds are discussed, and examples of cell morphology engineering importance of are shown.

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KEY WORDS: vitamin E biosynthesis, carotenoids, metabolic engineering, synthesis of shikimate pathway precursors

DOI: 10.1134/S0006297925603764

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