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Biochemistry (Moscow). Papers in Press. Published on April 29, 2019 as Manuscript BM19-011.

Protective Effect of Peroxiredoxin 6 Against Toxic Effects of Glucose and Cytokines in Pancreatic RIN-m5F β-Cells

E. G. Novoselova1,a*, O. V. Glushkova1, S. B. Parfenuyk1, M. O. Khrenov1, S. M. Lunin1, T. V. Novoselova1, M. G. Sharapov1, I. A. Shaev1, and V. I. Novoselov1

1Institute of Cell Biophysics, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russia

*To whom correspondence should be addressed.

Received January 14, 2019; Revised March 18, 2019; Accepted March 18, 2019
Taking into account a special role of pancreatic β-cells in the development of diabetes mellitus, the effects of peroxiredoxin 6 (Prx6) on the viability and functional activity of rat insulinoma RIN-m5F β-cells were studied under diabetes-simulating conditions. For this purpose, the cells were cultured at elevated glucose concentrations or in the presence of pro-inflammatory cytokines (TNF-αand IL-1) known for their special role in the cytotoxic autoimmune response in diabetes. It was found that the increased glucose concentration of 23-43 mM caused death of 20-60% β-cells. Prx6 added to cells significantly reduced the level of reactive oxygen species and protected the RIN-m5F β-cells from hyperglycemia, reducing the death of these cells by several fold. A measurement of insulin secretion by the RIN-m5Fβ-cells showed a significant stimulatory effect of Prx6 on the insulin-producing activity of pancreatic β-cells. It should be noted that the stimulatory activity of Prx6 was detected during culturing the cells under both normal and unfavorable conditions. The regulation of the NF-κB signaling cascade could be one of the mechanisms of Prx6 action on β-cells, in particular, through activation of RelA/p65 phosphorylation at Ser536.
KEY WORDS: peroxiredoxin 6, hyperglycemia, cytokines, RIN-m5F β-cells, insulin production, signaling cascade NF-κB