ADE6 Gene of Saccharomyces cerevisiae Yeast Encoding Formylglycinamidine-Ribonucleotide Synthetase. Cloning, Sequencing, and Analysis

Yu. V. Andreichuk,¹ V. D. Domkin,^1,2 T. A. Ryzhova,¹ V. N. Koulikov,¹ and T. R. Kostikova¹

¹Biological Institute, St. Petersburg State University, Oranienbaumskoe Shosse 2, Staryi Petergof, St. Petersburg, 198904 Russia; fax: (007-812) 427-73-10; E-mail: vddomkin@vddomkin.spb.ru

²To whom correspondence should be addressed.

Submitted January 30, 1997; revision submitted March 25, 1997.

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The ADE6 gene of Saccharomyces cerevisiae yeast encoding the enzyme formylglycinamidine-ribonucleotide (FGAM)-synthetase of de novo synthesis of purine nucleotides was cloned and sequenced. The gene encodes a protein consisting of 1358 amino acids. The flanking regions of 1208 (5´) and 728 bp (3´) were also sequenced. The nucleotide motif TGACTC inherent to the promotor regions of other purine genes of yeast was located (-276 bp) in the 5´-region of the gene. The amino acid sequence of the yeast FGAM-synthetase was found to contain repeats (Leu⁴³⁰-Ala⁶²⁰ and Pro⁸¹⁰-Ile¹⁰⁰⁰). Repeats of similar patterns of conserved amino acids were also detected in the structure of all other FGAM-synthetases. A homology of FGAM-synthetases with certain proteins of viruses from the Herpesviridae family was found.

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KEY WORDS: Saccharomyces cerevisiae yeast, ADE6 gene, biosynthesis of purine nucleotides, formylglycinamidine-ribonucleotide synthetase, intraprotein repeat.

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