Assessment of Dot-Blot ELISA Sensitivity on Membrane Sorbent Using Various Peroxidase Substrates

A. P. Bogoyavlensky,^1,2 I. E. Digel,¹ and V. E. Berezin¹

¹Institute of Microbiology and Virology, ul. Bogenbai Batyra 103, Almaty, 480100 Kazakhstan.

²To whom correspondence should be addressed.

Submitted February 18, 1997; revision submitted April 17, 1997.

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The sensitivity of the peroxidase reaction in dot-blot ELISA significantly depends on the substrate. The highest sensitivity is observed using benzidine and diamine--phenol combinations as the substrates due to the reaction of the coupled oxidation (NADI).

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KEY WORDS: ELISA, phenols, naphthols, aromatic amines.

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