Comparative Kinetic Study of D-Glucose Oxidation by Ruthenium(III) Compounds Catalyzed by FAD-Dependent Glucose Oxidase and PQQ-Dependent Glucose Dehydrogenase

E. V. Ivanova^1*, A. Yu. Ershov², V. Laurinavicius³, R. Meskus³, and A. D. Ryabov¹

¹School of Chemistry, Lomonosov Moscow State University, Moscow 119899, Russia; fax: (095) 254-2179; E-mail: ivanka@enzyme.chem.msu.ru

²Department of Chemistry, St. Petersburg State University, St. Petersburg 198904, Russia

³Institute of Biochemistry, ul. Mokslininku 12, Vilnius 2600, Lithuania

^* To whom correspondence should be addressed.

Received February 13, 2002; Revision received July 11, 2002

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The comparative kinetic study of two glucose oxidizing enzymes, FAD-dependent glucose oxidase and PQQ-dependent glucose dehydrogenase, is presented in the artificial electron transfer mediator system based on ruthenium(III) compounds. It is demonstrated that FAD-dependent glucose oxidase and PQQ-dependent glucose dehydrogenase follow Michaelis kinetics in the D-glucose/ruthenium(III) system. PQQ-dependent glucose dehydrogenase is more active than FAD-dependent glucose oxidase in the process of D-glucose oxidation by ruthenium(III) compounds, this being due to the different catalytic mechanisms of these enzymes.

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KEY WORDS: D-glucose, ruthenium compounds, ruthenacycles, PQQ-dependent GDH

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