Structure of Lipid A from the Marine gamma-Proteobacterium Marinomonas vaga ATCC 27119^T Lipopolysaccharide

N. V. Kapustina, I. N. Krasikova^*, V. V. Isakov, N. M. Gorshkova, and T. F. Solov'eva

Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, pr. 100-let Vladivostoku 159, Vladivostok 690022, Russia; fax: 7(4232) 31-4050; E-mail: innakras@piboc.dvo.ru

^* To whom correspondence should be addressed.

Received May 27, 2003

---

The chemical structure of a novel lipid A obtained as a major component on hydrolysis of LPS from the marine gamma-proteobacterium Marinomonas vaga ATCC 27119^T with 1% AcOH was determined. Using chemical analysis and NMR data, it was shown to be beta-1,6-glucosaminobiose 1-phosphate acylated with R-3-hydroxydecanoic acid (at position 3), and R-3-dodecanoyloxydecanoic (or R-3-decanoyloxydecanoic) acid and R-3-(R-3-hydroxydecanoyl)oxydecanoic acids (at the 2- or 2´-positions). The absence of a fatty acid at the 3´-position and a phosphoryl group at the 4´-position, and also the presence of R-3-acyloxyalkanoic acid with R-3-hydroxyalkanoic acid as the secondary acid are unique features distinguishing the M. vaga lipid A from other ones.

---

KEY WORDS: marine proteobacteria, Marinomonas vaga, LPS, lipid A

---