Comparative Characterization of Extracellular and Intracellular Hydrocarbons of Clostridium pasteurianum

T. V. Bagaeva^* and E. E. Zinurova

Department of Microbiology, Kazan State University, ul. Kremlevskaya 18, Kazan 420008, Russia; fax: (8432) 38-4487; E-mail: Tatiana.Bagaeva@ksu.ru

^* To whom correspondence should be addressed.

Received May 8, 2003; Revision received October 7, 2003

---

Extracellular and intracellular hydrocarbons produced by Clostridium pasteurianum VKM 1774 during cultivation on glucose-containing media in an argon atmosphere or in the presence of carbon dioxide and molecular hydrogen were analyzed by gas-liquid chromatography. Intracellular hydrocarbons were 50-55% (C₂₅-C₃₅) n-alkanes. Carbon dioxide and molecular hydrogen stimulated synthesis of extracellular hydrocarbons, which comprised 90-95% (C₁₁-C₂₄) n-alkanes.

---

KEY WORDS: hydrocarbons, intracellular, extracellular, Clostridium pasteurianum

---