Functional Relationship between Na/K-ATPase and NMDA-Receptors in Rat Cerebellum Granule Cells

A. Boldyrev^1*, E. Bulygina¹, O. Gerassimova¹, L. Lyapina¹, and W. Schoner²

¹Department of Biochemistry, Faculty of Biology, Lomonosov Moscow State University, Moscow 119992, Russia; fax: (7-095) 939-1398; E-mail: aaboldyrev@mail.ru

²Justus Liebich University, Giessen D-35932, Germany

^* To whom correspondence should be addressed.

Received June 19, 2003; Revision received September 17, 2003

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Activation of rat cerebellum granule cells by N-methyl-D-aspartate (NMDA, 10^-4-10^-3 M) results in progressive increase in reactive oxygen species (ROS) and suppression of the ouabain-sensitive part of Na/K-ATPase activity. When Na/K-ATPase was inhibited by high ouabain concentrations (10^-5-5*10^-4 M), an increase in stationary ROS level in neuronal cells was noted, this effect being attenuated by NMDA antagonists, MK-801 and D-AP5. It is concluded that in cerebellum neurons, ouabain-resistant Na/K-ATPase is responsible for suppression of intracellular level of ROS, which, in turn, inhibit ouabain-sensitive Na/K-ATPase.

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KEY WORDS: reactive oxygen species (ROS), Na/K-ATPase, glutamate receptors, NMDA

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