[Back to Number 9 ToC] [Back to Journal Contents] [Back to Biokhimiya Home page]

Positioning of a Nucleosome on Mouse Satellite DNA Inserted into a Yeast Plasmid Is Determined by Its DNA Sequence and an Adjacent Nucleosome

G. I. Kiryanov1*, L. N. Kintsurashvili2, L. V. Isaeva1, and M. G. Zakharova1

1Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russia; fax: (7-095) 931-7211; E-mail: gikmsu@mail.ru

2Biological Faculty, Tbilisi State University, Tbilisi 380060, Georgian Republic; fax: (99532) 30-5035

* To whom correspondence should be addressed.

Received March 3, 2004; Revision received April 16, 2004
It has earlier been shown that multiple positioning of nucleosomes on mouse satellite DNA is determined by its nucleotide sequence. To clarify whether other factors, such as boundary ones, can affect the positionings, we modified the environment of satellite DNA monomer by inserting it into a yeast plasmid between inducible GalCyc promoter and a structural region of the yeast FLP gene. We have revealed that the positions of nucleosomes on satellite DNA are identical to those detected upon reconstruction in vitro. The positioning signal (GAAAAA sequence) of satellite DNA governs nucleosome location at the adjacent nucleotide sequence as well. Upon promoter induction the nucleosome, translationally positioned on the GalCyc promoter, transfers to the satellite DNA and its location follows the positioning signal of the latter. Thus, the alternatives of positioning of a nucleosome on satellite DNA are controlled by its nucleotide sequence, though the choice of one of them is determined by the adjacent nucleosome.
KEY WORDS: nucleosome, positioning, mouse satellite DNA, yeast cells