Two Methods for Determination of Transketolase Activity

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I. A. Sevostyanova, O. N. Solovjeva, and G. A. Kochetov^*

Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992 Moscow, Russia; fax: (7-495) 939-3181; E-mail: kochetov@genebee.msu.su

^* To whom correspondence should be addressed.

Received November 21, 2005; Revision received January 17, 2006

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Two new optical methods for transketolase activity assay using only one substrate, xylulose 5-phosphate or glycol aldehyde, have been developed. For transketolase activity assay in the first method, it is necessary to add auxiliary enzyme, glyceraldehyde phosphate dehydrogenase. It is not needed in the second method. The range of transketolase concentration in the activity assay is 0.036-0.144 U/ml for the first method and 1.8-6.8 U/ml for the second one.

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KEY WORDS: transketolase, determination of activity, spectrophotometric method

DOI: 10.1134/S0006297906050154

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