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Isolation of Active Yeast Telomerase Protein Est3p and Investigation of Its Dimerization in vitro

Yu. S. Sharanov1,2*, E. M. Smekalova1, M. I. Zvereva1, and O. A. Dontsova1

1Chemical Faculty, Lomonosov Moscow State University, 119992 Moscow, Russia

2Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992 Moscow, Russia; fax: (495) 939-3181; E-mail: sharanov@genebee.msu.ru

* To whom correspondence should be addressed.

Received March 1, 2007; Revision received April 8, 2007
In this study we proposed a method for isolation of Est3p modified with various affinity tags, which is applicable for structural and functional studies, and investigated homo- and heterodimer formation with various recombinant forms of Est3p.
KEY WORDS: yeast telomerase, protein Est3p, dimerization

DOI: 10.1134/S0006297907070036