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Pentaspan Membrane Glycoprotein, Prominin-1, Is Involved in Glucose Metabolism and Cytoskeleton Alteration

Chang Yang1, Yanli Yang1, Nishith Gupta2, Xiaojun Liu1, Aibin He1, Lizhong Liu1, Jin Zuo1, Yongsheng Chang1*, and Fude Fang1*

1National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Peking Union Medical College and Chinese Academy of Medical Sciences, 5 Dong Dan San Tiao, Beijing 100005, P. R. China; fax: +86-10-6525-3005; E-mail: fangfd@vip.sina.com; changyongsheng@yahoo.com

2Institute of Molecular Parasitology, Humboldt University, Berlin-10115, Germany

* To whom correspondence should be addressed.

Received March 16, 2007; Revision received April 25, 2007
This study constitutes the first report revealing the participation of prominin-1 in glucose metabolism and cytoskeleton alteration. Upon stimulation with high glucose, the expression of prominin-1 was up-regulated with concomitant down-regulation of its phosphorylation. Prominin-1 mutated at its phosphorylation site inflicted a global change in the expression of several genes associated with glucose metabolism in L6 myotube cells. Further, the over-expression of prominin-1 promoted glucose uptake in these cells. Prominin-1 undergoes sustained repression of its expression during confluence and differentiation of L6 myotube cells. The expression of prominin-1 in the MDCK cell modulated cell morphology and promoted cellular migration. These data imply that prominin-1 is involved in glucose metabolism and may regulate cellular glucose through its effect on cytoskeleton.
KEY WORDS: prominin-1, CD133/AC133, glucose, cytoskeleton

DOI: 10.1134/S000629790708007X