Structural–Functional Characteristics of a New Bioregulator Isolated from Bovine Pigmented Epithelium Tissue

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V. P. Yamskova^1*, V. S. Skripnikova², A. A. Molyavka², A. P. Il’ina², M. S. Krasnov¹, D. V. Margasyuk², A. V. Borisenko², B. B. Berezin², E. S. Kuznetsova³, A. K. Buryak³, and I. A. Yamskov²

¹Koltzov Institute of Developmental Biology, Russian Academy of Sciences, ul. Vavilova 26, 119334 Moscow, Russia; fax: (499) 135-8012; E-mail: Yamskova-vp@yandex.ru

²Nesmeyanov Institute of Organoelemental Compounds, Russian Academy of Sciences, ul. Vavilova 28, 119991 Moscow, Russia; fax: (499) 135-5037; E-mail: Yamskov@mail.ru

³Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences, Leninsky pr. 31, 119991 Moscow, Russia; fax: (495) 952-5308; E-mail: tsiv@phyche.ac.ru

^* To whom correspondence should be addressed.

Received March 16, 2009; Revision received April 21, 2009

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A new bioregulator operating in ultralow doses corresponding to 10^–17 mg/ml has been isolated from tissue of pigmented epithelium of bovine eyes. It has been established that the functional basis of this bioregulator is a complex of a low molecular weight regulatory peptide (4372 Da) and a modulator consisting of a mixture of proteins with molecular weights of 14.980-66.283 kDa. It has been shown that the regulatory peptide is responsible for membranotropic activity of the bioregulator, and the modulator proteins are responsible for biological action in ultralow doses. The data demonstrate an interrelation between nanocondition of the bioregulator and its ability to show activity in ultralow doses.

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KEY WORDS: bioregulators, regulatory proteins, nanoparticles, ultralow doses

DOI: 10.1134/S0006297909090041

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