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Antimutagenic Activity of Mitochondria-Targeted Plastoquinone Derivative

V. A. Chistyakov*, M. A. Sazykina, A. A. Alexandrova, N. I. Belichenko, E. V. Mashkina, L. V. Gutnikova, P. V. Zolotukhin, and T. P. Shkurat

Research Institute of Biology, Southern Federal University, pr. Stachki 194/1, 344090 Rostov-on-Don, Russia; E-mail: vladimirchi@yandex.ru

* To whom correspondence should be addressed.

Received November 2, 2009
The ability of cationic plastoquinone derivative 10-(6′-plastoquinonyl) decyltriphenylphosphonium (SkQ1) to modify processes of spontaneous and induced mutagenesis was studied. It is shown that daily introduction of this compound into male Wistar rats in doses of 25 and 250 nmol/kg during two weeks decreases spontaneous level of chromosome aberrations in anaphase in the eye cornea from 0.39 ± 0.09 to 0.13 ± 0.08 and 0.14 ± 0.05, respectively. The level of 8-hydroxy-2′-deoxyguanosine in blood serum of the investigated animals decreases from 32.12 ± 1.55 to 25.90 ± 2.26 and 25.76 ± 1.50 ng/ml, respectively. These facts indicate that the decrease in spontaneous clastogenesis is caused by decreased level of DNA damage by endogenous reactive oxygen species. A higher dose of SkQ1 also decreases to control level chromosome aberrations caused by oxygen under pressure of 0.5 MPa for 60 min. It is also shown in experiments with bacterial biosensors that SkQ1 is able to efficiently protect cells against genotoxic effect of UV radiation at 300-400 nm.
KEY WORDS: SkQ1, penetrating cations, antioxidants, mutagenesis, 8-OH-dG, chromosome aberrations, DNA damage, hyperbaric oxygenation, UV radiation

DOI: 10.1134/S0006297910030028