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REVIEW: Mass Spectrometric Approaches to Study Enveloped Viruses: New Possibilities for Structural Biology and Prophylactic Medicine

L. V. Kordyukova* and M. V. Serebryakova

Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991 Moscow, Russia; fax: (495) 939-3181; E-mail: kord@belozersky.msu.ru

* To whom correspondence should be addressed.

Received March 7, 2012; Revision received April 20, 2012
This review considers principles of the use of mass spectrometry for the study of biological macromolecules. Some examples of protein identification, virion proteomics, testing vaccine preparations, and strain surveillance are represented. Possibilities of structural characterization of viral proteins and their posttranslational modifications are shown. The authors’ studies by MALDI-MS on S-acylation of glycoproteins from various families of enveloped viruses and on oligomerization of the influenza virus hemagglutinin transmembrane domains are summarized.
KEY WORDS: mass spectrometry, MALDI, enveloped viruses, influenza virus, hemagglutinin, S-acylation, transmembrane domain, proteomics, vaccine, strain surveillance

DOI: 10.1134/S0006297912080044