2Department of Thoracic and Cardiovascular Surgery, First Hospital Affiliated with the Medical College of Shihezi University, Shihezi 832008, Xinjiang, China
4Department of Cardiovascular Surgery, Henan Provincial People’s Hospital, Zhengzhou 450000, Henan, China
5Department of Cardiac Surgery, Jiangxi Provincial People’s Hospital, Nanchang 330006, Jiangxi, China
* To whom correspondence should be addressed.
Received August 23, 2013; Revision received March 28, 2014
The purpose of the present study was to examine changes in preadipocytes following the coculture of preadipocytes and adipocytes and the effects on the secretion of adipocytes and macrophages following induction of inflammation and insulin resistance. Mature adipocytes and RAW264.7 macrophages were treated with lipopolysaccharide and insulin to establish models of inflammation and insulin resistance, respectively. The mRNA expression levels of IL-6, MCP-1, and TNF-α in all adipocyte treatment groups were significantly greater compared with the control, and that of adiponectin was less (P < 0.05). In the RAW264.7 macrophages, the mRNA expression levels of IL-6 and TNF-α were greater than those in the control group (P < 0.05). Moreover, the results of this study confirmed that adipocytes and macrophages increased the secretion of inflammatory factors under conditions of induced inflammation and insulin resistance. In addition, 3T3-L1 adipocytes inhibited the proliferation and differentiation of preadipocytes when cocultured with adipocytes under conditions of inflammation and/or insulin resistance, and the phenotype of preadipocytes did not change.
KEY WORDS: insulin resistance, adipocytes, preadipocytes, adipokines