2Sechenov First Moscow State Medical University, ul. Trubetskaya 8, 119992 Moscow, Russia
* To whom correspondence should be addressed.
Received March 10, 2014; Revision received April 28, 2014
The primary objective of this study was to determine the role of β2 integrin α-subunit (CD11b) in the mechanism of human polymorphonuclear leukocyte (PML) priming by S or Re endotoxin glycoforms from Escherichia coli for fMLP-induced respiratory burst. Similar priming activity of S and Re endotoxin glycoforms for fMLP-induced reactive oxygen species (ROS) generation from primed PML was found. Anti-CD11b antibodies (clone ICRF 44) as well as isotype-matched immunoglobulin G1 (clone MOPC-21) do not influence the fMLP-induced ROS generation from unprimed PML. Antibodies against CD11b do not change fMLP-induced ROS generation from endotoxin-primed PML as well. The involvement of different isoforms of Fcγ receptors in fMLP-induced ROS generation from activated PML is proposed.
KEY WORDS: lipopolysaccharide (endotoxin), β2 integrins (CD11b/CD18), Toll-like receptor 4, Fcγ receptors, receptor cluster, immunoglobulins, anti-human CD11b antibody, reactive oxygen species