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REVIEW: Overview of Fusion Tags for Recombinant Proteins

E. N. Kosobokova*, K. A. Skrypnik, and V. S. Kosorukov

Blokhin Russian Cancer Research Center, 115478 Moscow, Russia; E-mail: ekkos@mail.ru

* To whom correspondence should be addressed.

Received June 11, 2015; Revision received August 17, 2015
Virtually all recombinant proteins are now prepared using fusion domains also known as “tags”. The use of tags helps to solve some serious problems: to simplify procedures of protein isolation, to increase expression and solubility of the desired protein, to simplify protein refolding and increase its efficiency, and to prevent proteolysis. In this review, advantages and disadvantages of such fusion tags are analyzed and data on both well-known and new tags are generalized. The authors own data are also presented.
KEY WORDS: fusion tags, chromatography, enhancement of protein solubility

DOI: 10.1134/S0006297916030019