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Tankyrase Activity in Organs and Tissues of Mice

A. N. Kuimov1*, A. S. Zhozhikashvili1, V. N. Manskikh2, L. V. Platonova3, and T. G. Dyuzheva3

1Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991 Moscow, Russia; fax: +7 (495) 939-3181; E-mail: kuimov@genebee.msu.su

2Institute of Mitoengineering, Lomonosov Moscow State University, 119991 Moscow, Russia; fax: +7 (495) 939-3181

3Sechenov First Moscow State Medical University, 119991 Moscow, Russia; E-mail: dtg679@gmail.com

* To whom correspondence should be addressed.

Received July 20, 2015; Revision received September 7, 2015
Tankyrase, one of the NAD+ ADP-ribosyltransferases, is a target for drugs developed for their anticancer and other pharmacological activities. We designed an assay for estimation of the inhibition or activation of the enzyme in preclinical studies. In mice, the highest specific activity of tankyrase was observed in thymus, spleen, pancreas, and bone marrow. In murine liver, tankyrase is active in ontogenesis and during reparative regeneration; however, the basal activity is hardly detectable in normal liver and most of other organs of adult animals. We suggest that tankyrase is a part of the tissue growth and repair machinery, while its age-dependent inhibition, when an organism stops growing, turns on phenoptosis.
KEY WORDS: ADP-ribosyltransferase, Essentiale N, hepatic stimulator substance, poly(ADP-ribosyl)ation, regeneration

DOI: 10.1134/S0006297916030081