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REVIEW: CRISPR/Cas Genome Editing in Filamentous Fungi

Aleksandra M. Rozhkova1,a* and Valeriy Yu. Kislitsin1

1Bach Institute of Biochemistry, Federal Research Centre “Fundamentals of Biotechnology”, Russian Academy of Sciences, 119071 Moscow, Russia

* To whom correspondence should be addressed.

Received September 16, 2020; Revised October 15, 2020; Accepted October 22, 2020
The review describes the CRISPR/CAS system and its adaptation for the genome editing in filamentous fungi commonly used for production of enzyme complexes, enzymes, secondary metabolites, and other compounds used in industrial biotechnology and agriculture. In the second part of this review, examples of the CRISPR/CAS technology application for improving properties of the industrial strains of fungi from the Trichoderma, Aspergillus, Penicillium, and other genera are presented. Particular attention is given to the efficiency of genome editing, as well as system optimization for specific industrial producers.
KEY WORDS: CRISPR/Cas, Trichoderma, Aspergillus, Penicillium, genome editing

DOI: 10.1134/S0006297921140091