Site-Specific Endonucleases RspLKI and RspLKII from Rhodococcus species LK2 are Isoschizomers of SphI and BamHI

E. V. Zabaznaya,¹ L. A. Zheleznaya,² and N. I. Matvienko^1,3

¹Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region, 142292 Russia; fax: (095) 924-0493; E-mail: protres@sovam.com

²Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142292 Russia; fax: (095) 135-6219.

³To whom correspondence should be addressed.

Submitted February 25, 1997; revision submitted April 3, 1997.

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Two site-specific endonucleases, RspLKI and RspLKII, have been isolated and purified to functional homogeneity from the soil bacterium Rhodococcus species LK2. RspLKI recognizes the 5´-GCATG^vC-3´ DNA sequence and RspLKII recognizes the 5´-G^vGATCC-3´ sequence (arrows indicate DNA cleavage sites). The isolated enzymes are class II site specific endonucleases and are isoschizomers of endonucleases SphI and BamHI, respectively.

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KEY WORDS: site-specific endonuclease, isoschizomer, phosphocellulose, heparin-Sepharose, hydroxyapatite.

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